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. 2014 Dec 29;9(12):e116052. doi: 10.1371/journal.pone.0116052

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© 2014 Belon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Adherence of the mgtC mutant strain to J774 macrophages after a 3-hr period of infection at 4°C, as compared to the WT strain. Results are normalized to 100% for the wild-type strain and expressed as means+SD from three independent experiments. (B) J774 macrophage internalization of the WT, the mgtC mutant and the complemented strains after a 3-hr period of infection in the presence of cytochalasin D (10 µg/ml). Results are normalized to 100% for the wild-type strain and expressed as means+SD from three independent experiments. (C) Internalization of the mgtC mutant and complemented strains in HeLa cells. The wbbl2 mutant strain is included as a positive control. Results are normalized to 100% for the wild-type strain and expressed as means+SD from four independent experiments. Asterisks indicate statistical significance (* P<0.05).