Table 1. Bacterial strains and plasmids.

Strains, plasmids, phages	Description or phenotype	Source/Ref.
Mycobacterium marinum strains
Mma M	wild-type	[16]
Mma ΔmgtC	ΔmgtC	This study
Mma ΔmgtC+mgtC	ΔmgtC+attB::mgtC	This study
Mma Δwbbl2	Δwbbl2	[26]
Mycobacterium smegmatis strains
mc2155	Electroporation-proficient ept mutant of M. smegmatis strain mc26	[49]
Plasmid
pJSC347	Vector for cloning allelic-exchange substrates to be used for specialized transduction, HygR	[50]
pMV306	Single-copy-integrating vector, KanR	[22]
pMV306-mgtC	pMV306 carrying the mgtC gene and 840 bp upstream, KanR	This study
pMV261-mCherry	pMV261 carrying mCherry under the control of the hsp60 promoter, ZeoR	[51]
Phage
phAE159	Conditionally replicating shuttle phasmid derived from the lytic mycobacteriophage TM4	Kind gift from W.R. Jacobs