Figure 3. Expression of the MHC-nLAC reporter transgene following 9 consecutive daily injections of NRG1β1.

Treated hearts were subjected to retrograde collagenase perfusion, and the resulting dispersed cell preparations were reacted with X-GAL and processed for cardiac alpha-actinin immune reactivity. Cardiac alpha-actinin immune reactivity (red signal) and nuclear β-galactosidase activity (inset) in a bi-nucleated (left panel) and a mono-nucleated (right panel) cardiomyocyte. Bar = 50 microns.