Table 1. Cardiomyocyte DNA synthesis in adult MHC-nLAC mice following vehicle or NRG1β1 injection.
| Experiment;Mouse Treatment;NucleotideDelivery Method | GeneticBkg.* | NRG1β1 µg/Injection | BrdU+or 3H-Thy+CMNuclei/Total | PositiveNuclei ±SEM (%) | # MiceAnalyzed | p vs.Control |
| Experiment #1;nine daily NRG1β1injections inuninjured mice;BrdU mini-pump | DBA | 0 | 36/187,169 | 0.019±0.005 | 7 | Control |
| DBA | 2.5 | 7/207,490 | 0.003±0.001 | 7 | <0.05 | |
| Experiment #2;nine daily NRG1β1injections inuninjured mice; 3H-Thy injection onday nine | DBA | 2.5 | 1/182,420 | 0.0005±0.0004 | 5 | Control |
| F1 | 2.5 | 1/216,192 | 0.0005±0.0006 | 3 | >0.05 | |
| Experiment #3;three dailyNRG1β1 injectionsin uninjured mice;3H-Thy injectionon day three | DBA | 7.5 | 2/383,919 | 0.0005±0.0002 | 3 | >0.05† |
| Experiment #4;seven dailyNRG1β1 injectionsin MI mice; 3HThy injectionon day seven | DBA | 0 | 9/23,181 | 0.039±0.011 | 5 | Control |
| DBA | 2.5 | 8/29,463 | 0.027±0.021 | 6 | >0.05 |
*Genetic background, DBA = DBA/2J; F1 = [C57Bl/6J×DBA/2J]F1.
†
>0.05 vs. mice receiving 9 injections of lower dose of NRG1β1, followed by a single injection of 3H-Thy.