Effects of inhibitors on uptake of fluorescein isothiocyanate-labeled nanoparticles on (A) the VK2/E6E7 cell line and (B) the End1/E6E7 cell line. Cells are incubated with nanoparticles for 24 h and lysed by 1% Triton™ X-100 (Sigma-Aldrich, MO, USA). Nanoparticles are prepared with a concentration of chitosan–thioglycolic acid conjugate (w/v%) of 1, a sodium tripolyphosphate/chitosan–thioglycolic acid conjugate weight ratio of 0.96 and a drug/chitosan–thioglycolic acid conjugate weight ratio of −1. Data are shown as the mean ± the standard error of the mean (n = 3).
*p < 0.05 verus control.
**p < 0.01 versus control.
PMA: Phorbolmyristate acetate.