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. 2014 Dec 29;9(12):e115839. doi: 10.1371/journal.pone.0115839

Figure 4. Growth and survival of E. faecalis OG1RF, Δrsh, ΔrelQ, and ΔrshΔrelQ in human (A) serum and (B) whole blood ex vivo.

Figure 4

Overnight cultures of the four strains were diluted 1∶20 in serum or whole blood and incubated at 37°C. Dilutions of aliquots collected at 0, 3 (panel B), 6 (panel A), 9, 24, 48, and 72 hours were plated to assess bacterial survival. Results are reported as percent survival with the 0 hour time point set to 100%. Values and error bars represent the mean ± SEM of n = 3 independent replicates. Data were analyzed for statistical significance with two-way ANOVA followed by Dunnett’s multiple comparisons test. *, p<0.05; **, p<0.01; ***, p<0.001.