Figure 4. The expression of phospho-EGFR is not affected during follicle differetiation in the iRhom2^Uncv mutation.

(A–D) Immunohistochemistry for phospho-EGFR in the dorsal skin of P3 and P5 wild-type and iRhom2^Uncv/Uncv mice. (E) Western blot analysis of phospho-EGFR and phospho-ERK expression in the dorsal skin of P3 wild-type and iRhom2^Uncv/Uncv mice. (F) 293T cells were transfected with the indicated constructs, and the cell lysates were then probed with anti-Flag, anti-Myc and anti-β-actin antibodies (white arrowhead is a non-specific band). Transfected cells were treated with 10 µM MG132 for 12 hours. Scale bars: (A–D), 25 µm.