Figure 1. Sequence for administration of therapy.

A) The heart was exposed as described in the Methods section under “surgical procedure”. B) The left anterior descending coronary artery is ligated with a permanent suture (arrow) and a SPY image is collected to visualize change in perfusion. C) Sutures are placed to designate the treatment sites. D) Sequence of treatment, applicator containing 4 electrodes and injection port is placed at the treatment site marked by suture; 100 µl of plasmid solution at a concentration of 2 mg/ml is injected; 8 pulses are administered. Procedure is repeated for the other 3 sites.