TABLE 3.
Sample no. |
A450 (nm)
|
% Inhibitionc | |
---|---|---|---|
Control seruma | Positive serumb | ||
1 | 0.97 | 0.239 | 76 |
2 | 0.196 | 0.087 | 66 |
3 | 0.253 | 0.092 | 64 |
4 | 1.728 | 0.229 | 87 |
5 | 1.907 | 0.132 | 94 |
6 | 0.434 | 0.119 | 73 |
7 | 2.209 | 0.356 | 84 |
8 | 0.422 | 0.08 | 81 |
N protein positive reaction serum (100 μl) was mixed with 20 μl of normal serum in each test.
N protein positive reaction serum (100 μl) was mixed with 20 μl of convalescent-phase serum from patients with SARS in each test.
Test samples were considered to be confirmed as positive for N protein if the percentage of inhibition was greater than 50.