Figure 2. Transgenically expressed HopZ1a in Arabidopsis zar1 background does not induce an HR.

(A) Phenotype of transgenic HopZ1a and HopZ1a^C/A in zar1-1 plants 24 hours after transgene induction by spraying with 30 µM dexamethasone. (B–C) Immunoblot analysis of HopZ (B) and HopZ^C/A (C) proteins expressed in transgenic lines after treatment with 30 µM dexamethasone or water. Transgenic HopZ1a is in a zar1-1 background while HopZ1c, HopZ2 and HopZ3 are in a Col-0 background. The Ponceau Red stained blot serves as the loading control. The expected sizes are as follows: HopZ1a-HA 42.1 kDa, HopZ1c-HA 30.5 kDa, HopZ2-HA 41.9 kDa, HopZ3-HA 46.9 kDa, and the expected band is marked with an asterisk.