Figure 4. Phylogenetic positions of fungal endo-β-1,4-xylanase (GH11) amino-acid sequences.
Fungal GH11 amino-acid sequences were deduced from the nucleotide sequences amplified from beech (BB) or spruce (BS) soil cDNAs. The Maximum-likelihood phylogenetic tree includes all Sanger sequences amplified from the 2007 soil cDNA samples (BS2007 and BB2007) and all non-singleton sequence clusters detected by Illumina MiSeq sequencing of the 2010 soil cDNA samples (BS2010 and BB2010). Representative Ascomycota and Basidiomycota sequences are marked in red and blue, respectively, whereas the environmental sequences appear in green. Stars identify reference sequences obtained in the present study. Robustness of the tree topology was tested by bootstrap analysis (1000 replicates) and only bootstrap values ≥80 are given.