Table 1. Degenerate primers used in this study.
| Gene family-Subfamily1 | Primer namesand sequences (5′-3′) | PCR fragmentlength (bp) | Targetedfungal groups | Reference |
| GH7 | fungcbhI-F: ACC AAY TGC TAY ACI RGY AA; fungcbhI-R: GCY TCC CAI ATR TCC ATC | 515 | Basidiomycota;Ascomycota | [23] |
| GH5-5 | fungGH5-5-F: GAR ATG CAY CAR TAC CTY GA; fungGH5-5-R: CA NGG ICC RGC RGC CCA CCA | 248 | Basidiomycota;Ascomycota | This study |
| GH11 | fungGH11-F: GGV AAG GGI TGG AAY CCN GG; fungGH11-R: TG KCG RAC IGA CCA RTA YTG | 281 | Basidiomycota;Ascomycota | This study |
| AA2 | basidioAA2-F: GGY GGI GGI GCB GAY GGY TC; basidioAA2-R: GG RGT IGA GTC RAA NGG | 398 | Basidiomycota | This study |
1
according to the CAZy database (http://www.cazy.org/; [34]). The GH7 family encodes essentially either fungal endo-β-1,4-glucanases (E.C. 3.2.1.4) or cellobiohydrolases (E.C. 3.2.1.176), both active on cellulose; the GH5-5 subfamily only encodes fungal endo-β-1,4-glucanases (E.C. 3.2.1.4); the GH11 family encodes only endoxylanases and almost exclusively fungal endo-β-1,4-xylanases (EC 3.2.1.8); The AA2 family comprises Manganese (MnP; EC 1.11.1.13), lignin (LiP; EC 1.11.1.14), versatile (VP; EC 1.11.1.16) and generic peroxidases (GP; 1.11.1.7).