Table 2. Illumina MiSeq sequencing results obtained for Breuil Spruce (BS2010) and Breuil Beech (BB2010) forest soils.
| BS2010 GH7 | BB2010 GH7 | BS2010 GH11 | BB2010 GH11 | BS2010 AA2 | BB2010 AA2 | |
| Total no. of sequences | 1068 | 490 | 6060 | 1382 | 1782 | 553 |
| No. of clusters including singletons | 115 | 38 | 84 | 23 | 102 | 19 |
| No. of singletons (%) | 67 (58%) | 26 (68%) | 53 (63%) | 11 (48%) | 60 (59%) | 10 (53%) |
| No. of sequences used in subsamples with (without) singletons | 490 (465) | 490 (465) | 1382 (1372) | 1382 (1372) | 553 (542) | 553 (542) |
| No. of clusters in subsamples with (without) singletons | 83 (41) | 38 (13) | 40 (24) | 23 (13) | 49 (39) | 19 (9) |
| Shannon index with (without) singletons 1 | 3.34 (3.01) | 1.87 (1.58) | 2.38 (2.27) | 1.43 (1.38) | 2.83 (2.77) | 1.69 (1.59) |
| Schao1 with (without) singletons 1 | 251 (44) | 103 (13) | 82 (31) | 41 (13) | 82 (48) | 64 (9) |
Sequence statistics, diversity indexes and richness estimators calculated on subsamples generated from the initial sequence datasets (including singletons) or from datasets without the singletons.
All functional genes encode lignocellulolytic enzymes, i.e. fungal cellulases (GH7), fungal endo-β-1,4-xylanases (GH11), and Basidiomycota class-II peroxidases (AA2).
1
Shannon indexes and Schao1 estimators were calculated using datasets, which contained identical number of sequences for both forest soils.