TABLE 3.
Primers used for multiplex PCR identification of the most frequent Salmonella first-phase flagellar antigens
| Primer | Sequence (5′-3′) | 5′ position in fliC genea | Reference or source |
|---|---|---|---|
| Sense-60 | GCAGATCAACTCTCAGACCCTGGG | 481-504 | 17 |
| Antisense-i | ATAGCCATCTTTACCAGTTCC | 714-734 | This work |
| Antisense-z10 | CGTCGCAGCTTCTGCAACC | 911-929 | This work |
| Antisense-b | CGCACCAGTCYWACCTAAGGCGG | 628-650 | This work |
| Antisense-eh | AACGAAAGCGTAGCAGACAAG | 658-678 | This work |
| Antisense-lv | CCTGTCACTTTCGTGGTTAT | 790-809 | This work |
| Antisense-r | AAGTGACTTTTCCATCGGCTG | 741-761 | This work |
| Forward-d | CCCGAAAGAAACTGCTGTAACCG | 539-561 | This work |
| Reverse-d | TGGATATCAGTATTGCTCTGGGC | 625-647 | This work |
| Forward-G | GTGATCTGAAATCCAGCTTCAAG | 547-569 | This work |
| Reverse-G | AAGTTTCGCACTCTCGTTTTTGG | 1055-1078 | This work |
| Forward-Sdf-l | TGTGTTTTATCTGATGCAAGAGG | — | 1 |
| Reverse-Sdf-l | CGTTCTTCTGGTACTTACGATGAC | — | 1 |
a
Dashes indicate that primers do not target the fliC gene.