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. 2014 Dec 29;9(12):e115278. doi: 10.1371/journal.pone.0115278

Figure 2. D4Z4 methylation analysis by bisulfite sequencing.

Figure 2

At least 30 clones were analyzed by Quantification Tool for Methylation Analysis (QUMA). The figure represents cumulative results for ≥10 independents clones sequenced in both directions. a. Scheme representing bisulfite primer location. b. Region B1 contains 30 CpGs dinucleotides. c. Region B2 contains 41 CpGs dinucleotides. d. Region B3 contains 23 CpGs dinucleotides. e. Region B4 contains 36 CpGs dinucleotides. f. Region B5 contains 21 CpGs dinucleotides. g. Region B6 contains 43 CpGs dinucleotides. h. Region B7 contains 25 CpGs dinucleotides. Red boxes highlight single non-methylated CpGs. Open Circle correspond to unmethylated CpG. Close circle correspond to methylated CpG dinucleotide.