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. 2014 Dec 29;9(12):e115300. doi: 10.1371/journal.pone.0115300

Figure 4. Overexpression of c-Myc impairs miR-33b-induced inhibition of proliferation and invasion in osteosarcoma cells.

Figure 4

(A) Western blot analysis of c-Myc protein expression in MG-63 cells co-transfected with either a miR-33b mimics or scramble and pCDNA-c-Myc or pCDNA empty vector; GAPDH was also detected as a loading control. (B) The CCK8 assay used to evalute the proliferation of MG-63 cells transfected with different combinations. (C) Invasion assay of MG-63 cells treated with different combinations. The ratio of invasive cells per field is shown right. LSD test was used to analyze the significant differences. *p<0.05,** p<0.01,***p<0.001.