Figure 3.

The expression of AChE is increased in the apoptosis cell induced by cisplatin. (A) MCF-7 cells were treated with 100 μM cisplatin for different time as indicated. And then AChE immunostaining and Hoechst staining were applied in the cells. (B) Inhibition of p53 or AChE expression attenuates the apoptosis induced by cisplatin. MCF-7 cells were treated with 100μM cisplatin for 24 h in the absence or presence of p53 or AChE siRNA. Then cells were conducted by Hoechst 33342 staining assay. The amount of apoptotic nuclei with condensed chromatin were counted from representative photomicrographs and were represented as a percentage of the total number of nuclei counted. Each treatment group was compared with the other groups using unpaired t test.