FIG 1.

Amino acid sequence alignment of native and synthesized forms of BNBD3. sBNBD3 (published sequence [UniProtKB/Swiss-Prot accession number P46161] with glutamine in positions 1 and 27), aBNBD3 (analog in which glycine replaces glutamine in positions 1 and 27), and nBNBD3 (native BNBD3 sequence, with pyroglutamic acid [pE], the modified residue of glutamine, at the N terminus) were aligned with murine BD2 (mBD2) and human BD2 (hBD2). Aligned conserved cysteine residues are shown in the shaded vertical bars, and the β-defensin disulfide bonds (Cys1-Cys5, Cys2-Cys4, and Cys3-Cys6) are shown at the top.