FIG 2.

Chemotaxis of bovine iDCs in response to native and synthesized forms of BNBD3. Bovine iDCs were labeled with calcein acetoxymethyl ester and placed on a filter membrane (5-μm pores) above triplicate wells filled with either medium or 0.01, 0.1, 1, 10, 100, or 1,000 ng/ml of each BNBD3 peptide. The migration of cells toward medium or defensin was determined by reading the calcein fluorescence signal of migrated cells on the bottom of the filter after incubation at 37°C for 90 min. Data are expressed as the chemotactic index (CI) and are shown as the mean CI ± standard error of the mean (sem) for four donor animals (n = 4). sBNBD3 indicates the published sequence with glutamine in positions 1 and 27; in aBNBD3, glycine replaces glutamine in positions 1 and 27.