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Western blot detection of three viral glycoproteins produced in sf9 cells. Lanes 1 to 5 show purification of the recombinant CSFV E2 protein (lane 1, cell lysate; lane 2, supernatant; lane 3, flow-through fraction; lane 4, wash; lane 5, elution fraction). Two Erns proteins of BVDV (lane 6) and CSFV (lane 7) were also purified in the similar way. Anti-His monoclonal antibody was used in the analysis.
