FIG 2.

Purification of S. boydii catalase A1. (A) Fractionation of the crude somatic extract by anion-exchange chromatography on DEAE-Trisacryl. The locations of the different catalases as evidenced by the spectrophotometric detection of the enzyme activity and native PAGE analysis of the corresponding fractions with negative staining are indicated on the chromatogram. (B) Hydrophobic interaction chromatographic fractionation of pooled catalase A1-containing fractions from anion-exchange chromatography. Fractions containing catalase A1 are indicated on the chromatogram. (C) Molecular size exclusion chromatographic fractionation of pooled catalase A1-containing fractions recovered from hydrophobic interaction chromatography. Fractions containing catalase A1 are indicated on the chromatogram. AU, arbitrary units.