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. 2014 Dec 30;22(1):37–45. doi: 10.1128/CVI.00482-14

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FIG 3 — PAGE analysis of catalase A1. (A) Double staining according to Wayne and Diaz (29) after native PAGE analysis of crude somatic extracts from A. fumigatus CBS 113.26 (lane 1) and S. boydii IHEM 15155 (lane 2). (B) Ferricyanide-negative staining of native 5 to 15% polyacrylamide gels loaded with S. boydii crude somatic extract (lane 3), unbound fraction from affinity chromatography on concanavalin A-Sepharose (lane 4), and fraction eluted from the column with 0.2 M methyl α-d-mannopyranoside (lane 5). (C) S. boydii crude somatic extract (lane 6) and purified catalase A1 (lane 7) probed with peroxidase-concanavalin A after SDS-PAGE and Western blotting.