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Effects of different reagents on catalase A1 activity
| Reagent (final concn) | Residual activity (%)a |
|---|---|
| Potassium cyanide (10 mM) | 0 |
| Sodium azide (10 mM) | 0 |
| 3-AT (4 mM) | 38 |
| Ethanol-chloroform (25%–15%) | 71 |
| Cu2+ (10 mM) | 52 |
| Hg2+ (10 mM) | 14 |
| SDS (4%) | 97 |
| 2-ME (30 mM) | 14 |
Residual activity was determined spectrophotometrically after incubation of the purified enzyme for 1 h in the presence of the different reagents tested.
