TABLE 2.

Flow cytometric analysis of CD3⁺ T cells and CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T-cell subsets from peripheral blood lymphocytes of immunized chickens^a

Treatment	% of peripheral blood lymphocytes in T cells with:
	CD3+	CD3+ CD4+ CD8−	CD3+ CD8+ CD4−
PBS	32.1 ± 2.12	14.5 ± 1.09	8.5 ± 1.56
ChIL-18 plasmid	49.3 ± 1.38b	21.7 ± 1.85b	17.2 ± 2.16b
Vaccine plus control plasmid	41.5 ± 1.52	14.9 ± 1.75	13.2 ± 1.65
Vaccine plus ChIL-18 plasmid	64.5 ± 2.17c	31.5 ± 2.23c	24.6 ± 1.62c
NDV vaccine	40.3 ± 1.39	15.2 ± 2.05	12.3 ± 2.21

^aChickens (n = 10) were euthanized on day 28 after the first immunization, and peripheral blood lymphocytes were collected for immunophenotyping. The data presented are means ± SD.

^bP < 0.05 compared with chickens immunized with the inactivated NDV vaccine.

^cP < 0.01 compared with chickens immunized with the inactivated NDV vaccine.