TABLE 2.
Concordance of results of gene-specific PCR with those of two established genomic fingerprinting methods in identifying CGA among 138 E. coli isolates
| CGA status by:
|
No. of isolates with pattern | No. of isolates with the following CGA status by gene-specific PCRa
|
||
|---|---|---|---|---|
| RAPD PCR | ERIC2 PCR | CGA (n = 63) | Non-CGA (n = 75) | |
| + | + | 62 | 62 | 0 |
| − | − | 75 | 0 | 75 |
| −b | + | 1b | 1 | 0 |
a
Gene-specific PCR was done with forward primer CGAf, which targets the C288T fumC polymorphism, and consensus reverse primer CGAr.
b
With four of five RAPD primers, ECOR strain 47 exhibited RAPD profiles highly similar to, but distinct from, those of the CGA reference isolates.