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Effect of SIT downregulation on silica incorporation in T. pseudonana frustules after cultures starved overnight were made replete with 20 μM Si(OH)4 (A and B) and 200 μM Si(OH)4 (C). Newly synthesized cell wall silica was measured by virtue of PDMPO labeling in a flow cytometer. Bars represent standard deviations (n = 3).
