FIG 3.

AC6 is localized to the tip of the trypanosome flagellum. (A) Western blot of protein extracts from 2913 cells and from cells expressing wild-type HA-tagged AC6 (AC6HA) or catalytically inactive AC6 (AC6**HA). Blots were probed with anti-HA antibody, or anti-tubulin antibody as a loading control. (B) Control cells (2913) or cells expressing HA-tagged wild-type AC6 (AC6HA) or the catalytic domain mutant (AC6**HA) were subjected to immunofluorescence with anti-HA antibody (green) and DAPI (blue). Bar, 2 μm.