Fig. 1.

Specificity of primers and amplicon length. The PCR amplification products for the eight reference genes were analyzed by agarose gel electrophoresis and dissociation curves. a PCR products were run on a 2 % agarose gel. A single band at the anticipate product size indicates that the PCR product is specific. M: size markers. b Dissociation curves for the PCR products. The single peak represents a specific PCR product