Fig. 2.

Binding characterization of T-0632, CCK, and benzodiazepine ligands. Shown are competition-binding curves for T-0632, CCK, BDZ-1, and L-364,718 to compete for binding of radioligand [¹²⁵I]T-0632 (A), [¹²⁵I]CCK (B), and [¹²⁵I]BDZ-1 (C) to membranes from CHO cells stably expressing the type 1 CCK receptor (CHO-CCK1R). Values represent percentages of maximal saturable binding that were observed in the absence of competitor. Data are expressed as means ± S.E.M. of duplicate determinations from three independent experiments. Shown also in parentheses are the IC₅₀ values ± S.E.M. in nanomolar units for each of the curves.