Fig. 6.

Identification of selectivity determinants for T-0632 binding using CCK1R/CCK2R TM site-mutant constructs. Shown are homologous competition curves for binding of T-0632 to membranes prepared from CHO cells expressing CCK1R/CCK2R TM constructs in which those CCK1R residues facing this pocket that are distinct in CCK1R and CCK2R were changed to those in CCK2R (Cawston et al., 2012; Harikumar et al., 2013). Values represent percentages of maximal saturable binding that were observed in the absence of competitor. Data are expressed as means ± S.E.M. of duplicate determinations from three independent experiments.