TABLE 1.
Nomenclature of CCK1R-CCK2R receptor chimeras
| Constructs | Nomenclature | Construct Sequences |
|---|---|---|
| TM site replacement constructs | CCK1R TM2 | CCK1R(N98T) [N2.61T]a |
| CCK1R TM3 | CCK1R(T117V,T118S) [T3.28V,T3.29S]a | |
| CCK1R TM6 | CCK1R(I329V,F330Y) [I6.51V,F6.52Y]a | |
| CCK1R TM7 | CCK1R(L356H) [L7.39H]a | |
| Exonic chimeric constructs | B1A2-5 | CCK2R(Met1-Arg50)-CCK1R(Glu38-Gln428) |
| B1-2A3-5 | CCK2R(Met1-Met134)-CCK1R(Gly122-Gln428) | |
| B1-3A4-5 | CCK2R(Met1-Thr217)-CCK1R(Trp209-Gln428) | |
| B1-4A5 | CCK2R(Met1-Leu269)-CCK1R(Arg251-Gln428) | |
| ECL2 chimeric constructs | CCK1R (ECL2 chimer 1) | CCK1R(Met1-Pro184)-CCK2R(Val198-Gln204)-CCK1R(Cys196-Gln428) |
| CCK1R (ECL2 chimer 2) | CCK1R(Met1-Pro184)-CCK2R(Val198-Arg214)-CCK1R(Gln207-Gln428) | |
| CCK2R (ECL2 chimer 3) | CCK2R(Met1-Pro197)-CCK1R(Phe185-Met195)-CCK2R(Cys205-Gly447) | |
| CCK2R (ECL2 chimer 4) | CCK2R(Met1-Pro197)-CCK1R(Phe185-Gln206)-CCK2R(Gln215-Gly447) |
a
Residues in brackets represent the nomenclature of Ballesteros and Weinstein (1992). The numbers in parentheses represent the amino acid codons in the WT receptor proteins, which include the signal sequences of each receptor because the specific sites of processing and removal for these receptors have not been established.