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. 2014 Oct 20;4(4):956–979. doi: 10.3390/biom4040956

Figure 3.

Figure 3

In the absence of GroEL beads, general FXN-p.Ile154Phe aggregation (2 µM) occurs in solution alone (no osmolytes) (A) in the presence of 4M Glycerol (B) or 1 M TMAO (C). FXN-p.Ile154Phe was incubated at 45 °C for 60 min in the absence and presence of the different osmolytes and the UV absorbance spectra at time 0 min and 60 min are represented. The spectra in the presence of 1 M TMAO shows a larger increase in light scattering contributions as assessed by the increase in the general baseline from general protein aggregation compared without osmolytes (A) or with glycerol (B). The 1 and 60 min spectra of FXN-p.Asp122Tyr under all three above conditions do not show a significant wavelength dependent shift in the baseline due to light scattering from aggregation (Supplementary Figure S3).