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. 2014 Dec 12;4(4):1093–1101. doi: 10.3390/biom4041093

Table 1.

Summary of chromatographic retention time and mass value of TMS derivatives.

Compound RT in GC (min) M+, GC/MS
TMS Derivatives
TMS Substitution Pattern
Daidzein 19.5 398 OH-group in position C7 (A-ring) and C4' (B-ring)
3',4'-ortho-dihydroxyisoflavone 24.1 486 OH-group in position C7 (A-ring), C3' and C4' (B-ring)
7,8-ortho-dihydroxyisoflavone 25.1 486 OH-group in position C7, C8 (A-ring) and C4' (B-ring)
6,7-ortho-dihydroxyisoflavone 26.7 486 OH-group in position C6, C7 (A-ring) and C4' (B-ring)
Glycitein 24.7 428 OH-group in position C7 (A-ring) and C4' (B-ring)