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(A) Immunocytochemistry of hNPC one day following transfection shows that transfected cells express high levels of the DMT1 protein when compared to wild-type cells. (B) After plating transfected and wild-type cells and fixing each day for 10 days, counts of DMT1 over-expressing cells reveal that DMT1 expression decreases toward background (wild-type) levels over the course of 10 days. Scale bar = 50 μm.
