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. 2014 Mar 26;5(21):10342–10355. doi: 10.18632/oncotarget.1867

Figure 3. NSC745885 treatment resulted in the down-regulation of EZH2 and G2/M cell-cycle arrest in bladder cancer cells.

Figure 3

(A) T24, MBT2, and SV-HUC-1 cells were treated with the indicated amount of NSC745885 for 24 h. (B) MGH-U1 and MGHUR cells were treated with the indicated amounts of NSC745885 and doxorubicin for 24 h. Whole cell lysates were harvested and prepared for Western blotting with the indicated antibodies. (C) The cells were treated with indicated dosages of NSC745885 and DMSO control (labeled as DMSO) for 24 h. The cell cycle was analyzed by FACS analysis (mean±SEM, n=3). The percentage of cells in the defined stage of cell cycle was determined by calculating DNA content histograms. The increased G2/M proportion was plotted in the barograph. Black, sub-G1 phase; white, G1 phase; light gray, S phase; dark gray, G2/M phase