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. 2014 Mar 26;5(21):10342–10355. doi: 10.18632/oncotarget.1867

Figure 5. Ectopic over-expression of EZH2 attenuated the growth inhibition effect of NSC745885.

Figure 5

(A) V5-tagged EZH2 tet-on conditional-over-expressing MBT2 stable transfectants were constructed and optimized for doxycycline induction. (B) V5-tagged EZH2 tet-on MBT2 cells were treated with or without 50 ng doxycycline. (C) V5-tagged EZH2 tet-on MBT2 cells were treated with indicated concentrations of NSC745885 in the presence or absence of 50 ng doxycycline. (D) PC3 cells transiently transfected with V5-EZH2 tet-on plasmid were treated with indicated concentrations of NSC745885 before and after doxycycline induction. All cell growth were measured by MTT assay (mean±SEM, n=3 for each group).