Figure 1. Variable effects of PI-3K/mTOR inhibitors on ALL cell proliferation and survival.

ALL cell lines (A) or patient derived stromal dependent cell lines (B) were cultured with the indicated concentration of the specified inhibitors for 24h and assessed for proliferation using ³H-thymidine. Data was normalized to control values and the mean±SD of quadruplicates are shown. ALL cell lines (C) or patient derived stromal dependent cell lines (D) were cultured with the indicated concentration of the specified inhibitors for 24h and assessed for viability by flow cytometry using annexin V and PI staining. Data was normalized to control values and the mean±SD of duplicates are shown. (E) Representative dot plots showing annexin V/PI staining following exposure to the indicated drug concentrations in NALM6 cells.