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. 2014 Jul 25;5(21):10460–10472. doi: 10.18632/oncotarget.2260

Figure 2. BEZ235 induces caspase-dependent cell death while BGT226 mediates a caspase independent cells death mechanism.

Figure 2

(A) Cells were exposed to 64 μM BEZ235, 1 μM BGT226 or 0.125 μg/ml doxorubicin for 24 h and caspase-3 cleavage assessed by intracellular flow cytometry. The mean ± SD of the percentage of positive cells from two replicate experiments are shown on each histogram. (B) The indicated cell lines were incubated with 10 mM Z-VAD for 60 min prior to the addition of vehicle alone (Control), Doxorubicin (0.86 μM), BEZ235 (72 μM for NALM6 and 100 μM for REH cells) or BGT226 (0.45 μM for NALM6 and 1 μM for REH cells) and the cells incubated for a further 16 h. Viability was assessed by annexin V/PI staining and mean ± SD of repeat experiments (2 ≤ n ≤ 5). *P<0.05.