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. 2014 Aug 27;5(21):10486–10502. doi: 10.18632/oncotarget.2395

Figure 5. Role of SCF in self-renewal growth of cancer stem-like side population cells.

Figure 5

(A) Sorting of SP and MP cells in H1650 cells by FACS analysis with Hoechst 33342 dye staining. (B, C) SCF regulates the self-renewal growth of side population cells (SP) and not main population cells (MP) from A549 and H1650 cells. Cell culture supernatants from A549 and H1650 cells transfected with control siRNA, ARRB1/β-arrestin-1 siRNA and stimulated with nicotine was used for sphere formation assay. There was a significant decrease in spheres after treatment with 0.5 and 1μg/ml SCF-neutralizing antibody. (D, E) Total number of spheres generated per well from 2000 cells is plotted.