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. 2014 Aug 27;5(21):10486–10502. doi: 10.18632/oncotarget.2395

Figure 6. Role of SCF and nicotine in self-renewal growth of cancer stem like side population cells.

Figure 6

(A) Sphere formation assay in SP and MP cells showing that nicotine independent of SCF can also regulate the self-renewal growth of SP cells but not as significant that of SCF that was confirmed by the addition of SCF neutralizing antibody that resulted in the marginal reduction in the number of spheres in SP cells in both A549 and H1650 cells. (B,C) Total number of spheres generated per well from 2000 cells is plotted. (D) Cell culture supernatants containing the secreted SCF with nicotine treated and untreated in A549 and H1650 cells were used for sphere formation assay in SP and MP cells. Human recombinant SCF (10ng/ml) was used to confirm the role of SCF on stem like SP cells. Results show that SP cells with nicotine induced SCF showed increased formation of tumor spheres and neutralization of SCF with the antibody completely abrogated the growth of spheres in SP. (E, F) Total number of spheres generated per well from 2000 cells is plotted.