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. 2014 Nov 4;5(21):10503–10517. doi: 10.18632/oncotarget.2391

Figure 4. PTEN-null isogenic MCF10A cells shows increased sensitivity towards combined treatment with metformin and erlotinib.

Figure 4

(A) Immunoblots confirming genetic alterations in the MCF10A isogenic lines. (B) Proliferation assays of parental, PTEN-null and ΔPTEN-dnp53-EGFR MCF10A cells treated with metformin (4 mM), erlotinib (4 μM) and their combinaton over 6 days. 6 replicates were used per treatment (* represents p < 0.03 and ** represents p < 0.0001). (C) Immunoblot analysis of EGFR, AKT and S6 signaling in parental and ΔPTEN-dnp53-EGFR MCF10A cells treated with metformin (4 mM), erlotinib (4 μM) and their combination for 1 hour.