Skip to main content
. 2014 Sep 25;5(21):10571–10583. doi: 10.18632/oncotarget.2512

Figure 3. SOX10represses tumor cell survival.

Figure 3

(A) SOX10 (green) was primarily located in the nucleus in SOX10-transfeced cells by immunostaining. DAPI counterstaining (blue) was used to visualize DNA. (B) Cells were infected with LV-SOX10 or LV-GFP and seeded in 96-well plates for MTS assay. Values are mean ± SD for triplicate samples from a representative experiment. *: p<0.05. **: p<0.01. (C) Representative colony formation assays. (D) Representative anchorage-independent growth. (E) Cells infected with LV-SOX10 or LV-Non were injected subcutaneously into nude mice. Tumor volume of each group was scored every 7 days (Upper). The expresson of SOX10 in the tumors was confirmed by IHC staining (lower). (F) In situ TUNEL apoptosis analysis was performed in tumor sections derived from the same mice as in Figure 3E. The apoptotic nuclei were seen as green color excited under fluorescence microscopy. (magnification ×400).