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. 2014 Sep 25;5(21):10571–10583. doi: 10.18632/oncotarget.2512

Figure 5. SOX10 suppresses Wnt signaling via compete with TCF4 to bind β-catenin.

Figure 5

Inhibition of TCF activity (A) and CCND1, c-Myc and MMP7 promoter reporter activities (B) in SOX10-expressing tumor cells in a dose-dependent manner. *: p<0.05, **: p<0.01. qRT-PCR (C) and western blot (D) showed ectopic expression of SOX10 disrupted Wnt signaling pathway. (E) Co-immunoprecipitation (Co-IP) assays showing that exogenous V5-SOX10 binds endogenous β-catenin and vice versa. IP antibody or IgG negative control was used as indicated. (F) GST pull-down assay was performed with GST-β-catenin fusion protein or GST and V5-SOX10 as indicated.