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. 2014 Sep 26;5(21):10678–10691. doi: 10.18632/oncotarget.2528

Figure 3. Expression of wild-type p53 but not mutant p53 restores VMY sensitivity.

Figure 3

(A) p53-null PC3 prostate cancer cells were transiently transfected with wild-type p53, p53-G245A or empty vector control. Cell viability was measured by trypan blue dye exclusion 18 hrs after treatment with VMY or purvalanol B (PVB) at the concentrations shown. (B) Western blots were performed for both p53 and for p21CIP1/WAF1 (p21) on extracts from PRIMA-1 and vehicle-control treated DU145 cells with or without 18 hrs exposure to 30uM VMY. (C) DU145 cells were treated with 75 uM PRIMA-1 and VMY or PVB for 18 hrs. Cell viability was measured by trypan blue dye exclusion (*, p<0.05, vs PRIMA-1 plus DMSO). (D) Western blots for p53 and the full-length PARP protein (PARP-FL) and the long form of activated, cleaved PARP (PARP-L). β-actin was used as a loading control. Western blotting was performed on extracts derived from the cells used in the experiments shown in panel C.