Figure 3. PI3Kδ inhibition led to blockade of B cell leukemia cells proliferation via induction of cell cycle arrest and apoptosis.

Raji cells treated with X-370 for indicated times and at serial diluted concentrations were subjected to Annexin V-FITC/PI staining (A) and PI staining (B) and FACS analysis to show cell apoptosis and cell cycle distribution respectively. (C) X-370 inhibited proliferation of human leukemia primary cells. Freshly isolated human primary mononuclear cells from bone marrow samples of patients were treated with X-370 for 72 h and cell viability was assayed using CCK-8 assay. IC₅₀s of each sample were plotted where 10 μM was assigned to specimens with IC₅₀ >10 μM. Each symbol represents an individual patient sample.