Figure 5. Effects of in vivo treatment on osteosarcoma.

(A) Tumors were measured with caliper every two days, starting on the 7^th day. Treatment with DAC was administrated from the 7^th day to the 11^th day, and mice started to receive i.v. injection of CTA specific T-cells every two days from the 12^th day. In mice pre-treated with DAC, T-cells represented high efficiency in inhibiting tumor growth. Statistical difference in tumor volume was shown the 17^th day, while there was no statistical difference among the other 3 groups. (B) Weights of the xenografts from (C) on the 19^th day. (C) Xenografts excised from the tumor bearing mice on day 19. (D) Western blot showed that expression of MAGE-A and NY-ESO-1 was elevated after in vivo treatment with DAC. (+) : Treated with DAC; (−) : Without DAC treatment. Error bars in (A) and (B) refer to Standard Error of Mean.