Skip to main content
. 2014 Oct 11;5(21):10886–10900. doi: 10.18632/oncotarget.2540

Figure 5. ITCH knockdown inhibits tumorigenicity of MDA-MB231 breast cancer cells.

Figure 5

(A) XTT proliferation assay showing the effect of ITCH knockdown on MDA-MB231 cell growth rate. (B) Quantification of cell survival rates using colony formation assay. (C) Quantitative presentation of Boyden Chamber Matrigel invasion assay results of MDA-MB231 cells expressing either empty vector (EV) or ITCH Sh construct. (D) Representative micrographs of Boyden Chamber Matrigel invasion assay of MDA-MB231 cells expressing either EV or ITCH Sh construct. (E) Micrographs of 3D cell culture of MDA-MB231 cells expressing either EV or ITCH Sh construct. (F) Real time PCR showing the effect of ITCH knockdown on YAP target genes. (G) Representative micrographs of lungs obtained from Nod-SCID mice injected into the tail vein (IV) with control or ITCH Sh MDA-MB231 breast cancer cells constitutively expressing GFP. Mice were analyzed 4-weeks post injection. (H) Graphical representation of the number of metastatic foci obtained in the lungs of mice in the IV model; n=6/group. In all figures, error bars represent the standard deviation of three different biological experiments done in triplicates. The statistical significance was measured by calculating the p values for all experiments related to EV. (*P value <0.05, **P value <0.01, ***P value <0.001).