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. 2014 Oct 11;5(21):10886–10900. doi: 10.18632/oncotarget.2540

Figure 6. YAP knockdown rescues ITCH-mediated EMT phenotypes in MCF10A cells.

Figure 6

(A) qRT-PCR results showing successful YAP mRNA knockdown accompanied with downregulation of YAP target gene mRNA in 50% cell confluent culture. (B) Colony formation assay results showing the effect of YAP silencing on MCF10A cells overexpressing ITCH. (C) Immunoblot analysis showing the effect of YAP knockdown on EMT markers induced by ITCH overexpression. (D) 3D culture of MCF10A cells expressing either ITCH alone or ITCH and YAP Sh construct. Arrows point to abnormal structures in the mammospheres. (E) Representative pictures of the invading cells of the different indicated clones in Boyden chamber Matrigel invasion assy. (F) Quantification of Boyden chamber Matrigel invasion assay results in E. In all figures, error bars represent the standard deviation of three different biological experiments done in triplicates. The statistical significance was measured by calculating the p values for all experiments comparing ITCH to EV, ITCH-YAP-Sh to ITCH. * indicates P value <0.05, **P value <0.01, ***P value <0.001.