Figure 5. Reduction of cell growth and colony formation ability in U251 and A172 cells by BRCC3 knockdown.

The stable transfectants (mock, BRCC3-KD1 and BRCC3-KD2) of U251 and A172 cells were seeded at a density of 5×10³ cells per well and subjected to MTT proliferation assay (A) and colony formation assay (B) as described in Materials and Methods. Data are means ± SEM of three independent experiments. *p < 0.05, **p < 0.01, versus mock (A and B). Scale bar in B, 5 mm.