Figure 10. Interaction of CXCL4^47–70 and CXCL4L1^47–70 with CCL5.

Changes in the expression of murine (mu) CCL5 within the tumor microenvironment were evaluated in vehicle (CO)-, CXCL4^47–70- and CXCL4L1^47–70-treated MDA-MB-231 tumors by qPCR (A). Fold changes in mRNA levels were calculated according the 2^−ΔΔCT method. The median value per group is indicated by a horizontal bar. In order to further evaluate a possible interaction between CCL5 and the administered peptides, binding of biotinylated CXCL4^47–70 or CXCL4L1^47–70 to human CCL5-coated plates was quantified (B), whereas functional synergy was investigated by evaluating chemotaxis of monocytic cells (C). THP-1 cells were stimulated with CCL5 (20 ng/ml), intact CXCL4 (500 ng/ml), CXCL4^47–70, CXCL4L1^47–70 (both 1000 to 5000 ng/ml) or combinations thereof in a multiscreen chemotaxis assay. A: n= 7 to 9; B: n= 3; C: n= 3 to 4; *p<0.05